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International Conference and Expo on Dermatopathology and Skin Care

Toronto, Canada

Dr. Hana Zelenkova

Dr. Hana Zelenkova

Private Clinic of Dermatovenereology, Svidnik, Slovakia

Title: Knowledge of the use of fibroblasts and keratinocytes in dermatology


Biography: Dr. Hana Zelenkova


Fibroblasts and keratinocytes represent great and popular therapeutic modalities used in medicine, including dermatology. In this respect, keratinocytes are very important in the treatment of skin defects especially in extensive burns. There are autologous and allogenic skin grafts. The development is heading towards the utilisation of biosynthetic and combined skin grafts that resemble the structure of normal skin –cultivated allogenic and autologous keratinocytes, allogenic or autologous composites, acellular matrices, collagen/hyaluronic acid based matrices, and matrices combining various types of cells (keratinocytes, dermal fibroblasts, stem cells). In a successful clinical practice, it is inevitable to establish and maintaingreat cooperation between the physician and an accredited tissue bank or a specialised laboratory. We shall present the usage of fibroblasts in the therapy of a huge pressure ulcer, with great regenerative and rejuvenating effects. Thesuspension of fibroblasts in salinesolutionwas administered in form of intradermal injections. Thenumber of injectionswasdetermined by thenature and size of theproblemarea on the skin. The anti-ageingtreatmentincluded 3 sessions. Theprocedurewasminimum invasive, no allergicreactionswereobserved. Great effects were documented in the usage of dermal and epidermal grafts in the therapy of acute and chronic wounds.